Morphological investigation of connective tissue dysplasia in pediatric surgery

The study determined accessible and highly specific morphological research techniques for diagnosing the severity of the connective tissue dysplasia (CTD) in children with hernias of the anterior abdominal wall. The main group consisted of 54 children with unilateral inguinal hernia, which had CTD of moderate or severe degree. The control group was formed of 36 patients with unilateral hydrocele, who had no CTD or had mild CTD. Children underwent a standard correction of the hernia or hydrocele with taking hernia sac / vaginal processus of the peritoneum. Operating material was studied morphologicaly: silvering by Foote, three-colour staining by Masson, immunohistochemical reaction to antibodies of Collagen I and IV Type. The study of morphological changes revealed the presence of significant changes (χ2 = 41.3, p = 0.001) in the structural changes of the connective tissue of the patients in the main and control group. For the morphologic diagnosis of CTD in children with inguinal hernia immunohistochemical study of collagen I and IV is the most reliable method. At the same time, technique of silver impregnation by Foote and three-colour staining by Mason has high diagnostic informative value during routine histological examination.

doi: 10.14300/mnnc.2016.11070


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